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[iTRAQ] 通过iTRAQ等技术分析叶绿素缺乏的茶树突变体的多重代谢途径

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发表于 2015-12-20 19:50:14 | 显示全部楼层 |阅读模式

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植物叶绿素缺失突变体在自然界中广泛存在,是研究叶绿素形成和叶 绿体发育等代谢途径的良好材料。但在前人的研究中,仅有少量的蛋白质组学数据,对于揭示叶绿素缺乏突变体的根本机制作用有限,而本文创新地联合运用互补转录组学、蛋白质组学、形态学等方法解释了其中几个重要的代谢通路——生物合成通路、谷胱甘肽代谢、苯基丙氨酸代谢、光合作用以及类黄酮生物合成通路。同时,通过微阵列芯片以及iTRAQ分析,差异表达的基因及蛋白也可反映在以上几条通路中。

     本文的研究对象是绿茶品种LJ43以及黄茶品种ZH1。从实验结果看来,ZH1LJ43相比具有更高含量的茶氨酸、游离氨基酸,而叶绿素、类胡萝卜素及儿茶酸含量则相对较少。

     意义:茶叶中各种化学成分如叶绿素、类胡萝卜素、游离氨基酸等是决定茶叶质量的关键因素。本文使用的新方法可以更好地让我们了解其中的分子机制,对培育出高质量变异品种具一定指导意义。

1.JPG 2.JPG


此图为LJ43与ZH1种株各种指标含量对比

3.JPG


A图为差异表达的基因分析

4.JPG

B图为5944个差异表达基因映射的通路

5.JPG 6.JPG

此两图为生物合成途径示意图



Abstract

To uncover themechanisms that underlie the chlorina phenotype of the tea plant,
this study employsmorphological, biochemical, transcriptomic, and iTRAQ-based
proteomic analysesto compare the green tea cultivar LJ43 and the yellow-leaf tea
cultivar ZH1. ZH1exhibited the chlorina phenotype, with significantly decreased
chlorophyllcontent and abnormal chloroplast development compared with LJ43. ZH1
also displayedhigher theanine and free amino acid content and lower carotenoid
and catechincontent. Microarray and iTRAQ analyses indicated that the
differentiallyexpressed genes and proteins could be mapped to the following
pathways: 'phenylpropanoidbiosynthesis,' 'glutathione metabolism,' 'phenylanine
metabolism,''photosynthesis,' and 'flavonoid biosynthesis.' Altered gene and
protein levels inthese pathways may account for the increased amino acid content
and reducedchlorophyll and flavonoid content of ZH1. Altogether, this study
combinestranscriptomic and proteomic approaches to better understand the
mechanismsresponsible for the chlorina phenotype.BIOLOGICAL SIGNIFICANCE:
Chlorina tea plantbreeding has recently become a topic of great interest.
Chlorina plantleaves exhibit altered levels of chloropyll, carotenoid, catechin,
and amino acids,all of which are important determinants of the quality of tea
and its healtheffects. However, partially due to insufficient proteomic data,
there is verylimited understanding of the underlying mechanism for this altered
phenotype. Here,we present a combined physiological, transcriptomic, and
proteomic analysisthat reveals several important pathways associated with the
chlorina tea plantphenotype. This integrative analysis provides a better
understanding ofthe molecular mechanisms that are responsible for the chlorina
phenotype and mayhave applications in the breeding of new, high-quality tea
cultivars.








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不明菲行物
不明菲行物  发表于 2015-12-21 22:27:27
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