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[iTRAQ] 乙肝病毒-iTRAQ-HepG2细胞

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发表于 2016-1-26 15:23:55 | 显示全部楼层 |阅读模式

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[size=0.8465em]PLoS One. 2014 Apr 24;9(4):e95621. doi: 10.1371/journal.pone.0095621. eCollection 2014.
Proteomics based identification of cell migration related proteins in HBV expressing HepG2 cells.IF=
14年3.234
13年3.534
12年3.73
11年4.092
研究关键词 乙肝病毒,Label-free,HepG2细胞
研究概要:
图片.png

研究目的:
通过蛋白质组学研究感染乙肝病毒的HepG2细胞特异蛋白表达谱,旨在鉴别位于HBx的SH3结合域(脯氨酸的特定功能发达地区)。
研究结论:
通过定量蛋白组学,感染细胞系中700个蛋白被鉴定。其中,与细胞迁移相关的蛋白收到HBV基因组的严重干扰。通过引入突变HBV转染的细胞系,初步界定在HBx上的SH3结合区域参与这一过程。
案例选取要点:
典型医学类iTRAQ蛋白组学试验设计:iTRAQ-3标,对照组与实验组分别5个重复,差异蛋白组交集满足包含于至少3个重复;
数据分析类型:囊括常规差异蛋白组学生物信息分析。


Abstract
Proteomics study was performed to investigate the specific protein expression profiles of HepG2 cells transfected with mutant HBV compared with wildtype HBV genome, aiming to identify the specific functions of SH3 binding domain (proline rich region) located in HBx. In addition to the cellmovement and kinetics changes due to the expression of HBV genome we have observed previously, here we further targeted to explore the specific changes of cellular proteins and potential intracellular protein interactions, which might provide more information of the potential cellular mechanism of the differentiated cell movements. Specific changes of a number of proteins were shown in global protein profiling in HepG2 cells expressing wildtypeHBV, including cell migration related proteins, and interestingly the changes were found recovered by SH3 binding domain mutated HBV. The distinctive expressions of proteins were validated by Western blot analysis.

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