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[iTRAQ] 鲍曼不动杆菌-iTRAQ-Biomarker

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发表于 2016-1-26 16:27:27 | 显示全部楼层 |阅读模式

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BMC Genomics. 2015 May 30;16:422. doi: 10.1186/s12864-015-1608-z.
Quantitative proteomic analysis of host--pathogen interactions: a study of Acinetobacter baumannii responses to host airways.
IF=
14年3.986
13年4.041
12年4.397
11年4.073
研究关键词
鲍曼不动杆菌,iTRAQ,Biomarker


研究概要:
图片.png

研究目的:
对不同条件下鲍氏不动杆菌蛋白组进行定量分析,以揭示鲍氏不动杆菌侵染宿主机制以及获得该菌的病理与毒理相关特性。
研究结论:
获得179个差异表达蛋白,其中2个上调细胞壁合成相关蛋白与鲍氏不动杆菌侵染相关。该研究首次获得来自体内的鲍氏不动杆菌蛋白组信息,为开发诊断biomarkers、鉴定药物靶标打下基础。
案例选取要点:
典型iTRAQ蛋白组学试验设计:iTRAQ-4标,对照组与实验组分别3个重复,同时利用MASCOT对3个重复进行运算统计;
数据分析类型:囊括常规差异蛋白组学生物信息分析。


Abstract
BACKGROUND:
Acinetobacter baumannii is a major health problem. The most common infection caused by A. baumannii is hospital acquired pneumonia, and the associated mortality rate is approximately 50%. Neither in vivo nor ex vivo expression profiling has been performed at theproteomic or transcriptomic level for pneumonia caused by A. baumannii. In this study, we characterized the proteome of A. baumannii under conditions that simulate those found in the airways, to gain some insight into how A. baumannii adapts to the host and to improve knowledge about the pathogenesis and virulence of this bacterium. A clinical strain of A. baumannii was grown under different conditions: in the presence of bronchoalveolar lavage fluid from infected rats, of RAW 264.7 cells to simulate conditions in the respiratory tract and in control conditions. We used iTRAQ labelling and LC-MALDI-TOF/TOF to investigate how A. baumannii responds on exposure to macrophages/BALF.
RESULTS:
179 proteins showed differential expression. In both models, proteins involved in the following processes were over-expressed: (i) pathogenesis and virulence (OmpA, YjjK); (ii) cell wall/membrane/envelope biogenesis (MurC); (iii) energy production and conversion (acetyl-CoA hydrolase); and (iv) translation (50S ribosomal protein L9). Proteins involved in the following were under-expressed: (i) lipid metabolism (short-chain dehydrogenase); (ii) amino acid metabolism and transport (aspartate aminotransferase); (iii) unknown function (DNA-binding protein); and (iv) inorganic ion transport and metabolism (hydroperoxidase).
CONCLUSIONS:
We observed alterations in cell wall synthesis and identified 2 upregulated virulence-associated proteins with >15 peptides/protein in both ex vivo models (OmpA and YjjK), suggesting that these proteins are fundamental for pathogenesis and virulence in the airways. This study is the first comprehensive overview of the ex vivo proteome of A. baumannii and is an important step towards identification of diagnostic biomarkers, novel drug targets and potential vaccine candidates in the fight against pneumonia caused by A. baumannii

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