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[iTRAQ] 皮肤SDS刺激反应-iTRAQ

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发表于 2016-1-26 16:42:31 | 显示全部楼层 |阅读模式

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PLoS One. 2014 May 21;9(5):e97772. doi: 10.1371/journal.pone.0097772. eCollection 2014.
Proteomic analysis of the human skin proteome after in vivo treatment with sodium dodecyl sulphate
IF=
14年3.234
13年3.534
12年3.73
11年4.092
研究关键词 ,SDS,iTRAQ,皮肤刺激
研究概要:
图片.png


研究目的:
利用定量蛋白组学,探究SDS对人皮肤刺激的响应。
研究结论:
这项研究在领域首次对这一刺激从蛋白质组水平进行研究,653个皮肤蛋白被鉴定,然而差异比较蛋白组分析显示,皮肤在4hSDS刺激下,其蛋白组内蛋白量并无显著差异。这为进一步的研究提供了宝贵资源,尤其对长期皮肤与刺激物接触的过程的研究。
案例选取要点:
传统iTRAQ蛋白组学,对蛋白组进行全谱(shotgun)测定,且SDS-page与LC-MS/MS联用。
样本来源于医院收集病人样本。试验设计采用iTRAQ4标或8标,对n个样品(来源于医院志愿者)进行皮肤蛋白组定量,最终鉴定到找到皮肤总蛋白,结合总蛋白鉴定,分析皮肤应激SDS蛋白组变化机制。是一篇定量蛋白组方法论以及蛋白组学研究相结合的案例。

AbstractBACKGROUND:
Skin has a variety of functions that are incompletely understood at the molecular level. As the most accessible tissue in the body it often reveals the first signs of inflammation or infection and also represents a potentially valuable source of biomarkers for several diseases. In this study we surveyed the skin proteome qualitatively using gel electrophoresis, liquid chromatography tandem mass spectrometry (GeLC-MS/MS) and quantitatively using an isobaric tagging strategy (iTRAQ) to characterise the response of human skin following exposure to sodium dodecyl sulphate (SDS).
RESULTS:
A total of 653 skin proteins were assigned, 159 of which were identified using GeLC-MS/MS and 616 using iTRAQ, representing the most comprehensive proteomic study in human skin tissue. Statistical analysis of the available iTRAQ data did not reveal any significant differences in the measured skin proteome after 4 hours exposure to the model irritant SDS.
CONCLUSIONS:
This study represents the first step in defining the critical response to an irritant at the level of the proteome and provides a valuable resource for further studies at the later stages of irritant exposure.

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