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[iTRAQ] 脓毒血症-iTRAQ-biomarker

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发表于 2016-1-26 17:03:25 | 显示全部楼层 |阅读模式

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PLoS One. 2013;8(1):e54237. doi: 10.1371/journal.pone.0054237. Epub 2013 Jan 23.
Identification of novel biomarkers for sepsis prognosis via urinary proteomic analysis using iTRAQ labeling and 2D-LC-MS/MS.
IF=
14年3.234
13年3.534
12年3.73
11年4.092
研究关键词 ,脓毒血症,iTRAQ,biomarker
研究概要:
图片.png
图片.png

研究目的:
为脓毒症病人的早期诊断筛选潜在生物标记物。
研究结论:
总共232个蛋白被鉴定,其中,在差异蛋白中筛选可作为预断脓毒症的蛋白:5个成员显著上调,两个成员显著下调;通过基因GO聚类注释,这些蛋白参与脂代谢、软骨发育、铁离子转运等生物过程。尿LAMP-1蛋白显著下调,并通过western验证初步认为该蛋白可作为脓毒症预诊断的生物标记蛋白。

AbstractOBJECTIVES:
Sepsis is the major cause of death for critically ill patients. Recent progress in proteomics permits a thorough characterization of the mechanisms associated with critical illness. The purpose of this study was to screen potential biomarkers for early prognostic assessment of patients with sepsis.
METHODS:
For the discovery stage, 30 sepsis patients with different prognoses were selected. Urinary proteins were identified using isobaric tags for relative and absolute quantitation (iTRAQ) coupled with LC-MS/MS. Mass spec instrument analysis were performed with Mascot software and the International Protein Index (IPI); bioinformatic analyses were used by the algorithm of set and the Gene Ontology (GO) Database. For the verification stage, the study involved another 54 sepsis-hospitalized patients, with equal numbers of patients in survivor and non-survivor groups based on 28-day survival. Differentially expressed proteins were verified by Western Blot.
RESULTS:
A total of 232 unique proteins were identified. Proteins that were differentially expressed were further analyzed based on the pathophysiology of sepsis and biomathematics. For sepsis prognosis, five proteins were significantly up-regulated: selenium binding protein-1, heparan sulfate proteoglycan-2, alpha-1-B glycoprotein, haptoglobin, and lipocalin; two proteins were significantly down-regulated: lysosome-associated membrane proteins-1 and dipeptidyl peptidase-4. Based on gene ontology clustering, these proteins were associated with the biological processes of lipid homeostasis, cartilage development, iron ion transport, and certain metabolic processes. Urinary LAMP-1 was down-regulated, consistent with the Western Blot validation.
CONCLUSION:
This study provides the proteomic analysis of urine to identify prognostic biomarkers of sepsis. The seven identified proteins provide insight into the mechanism of sepsis. Low urinary LAMP-1 levels may be useful for early prognostic assessment of sepsis.
TRIAL REGISTRATION:
ClinicalTrial.gov NCT01493492.

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