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[其他蛋白质组] A549细胞-2D-HEV

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发表于 2016-1-26 18:12:02 | 显示全部楼层 |阅读模式

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BMC Vet Res. 2014 Aug 30;10:188. doi: 10.1186/s12917-014-0188-5.
Changes in the cellular proteins of A549 infected with hepatitis E virus by proteomics analysis.


IF=
14年1.777
13年1.743
12年1.861
14年2
研究关键词 A549细胞,2D,HEV

研究概要
图片.png


研究目的:
利用基于凝胶的蛋白质组学手段,研究A549细胞蛋白体外接触戊肝病毒的变化,以揭示HEV侵染A549细胞机制。
研究结论:
获得2585-3152个蛋白点,其中31个蛋白点为差异表达蛋白点,包括10个显著上调蛋白点,21个显著下调蛋白点。该研究首次获得细胞响应HEV蛋白组,为研究HEV侵染宿主分子机制打下基础。
案例选取要点:
传统的2D-gel联合打点质谱鉴定蛋白组学分析。
数据分析类型: 常规差异蛋白组学生物信息分析。

AbstractBACKGROUND:
Our understanding of Hepatitis E virus (HEV) has changed enormously over the past 30 years, from a waterborne infection causing outbreaks of acute hepatitis in developing countries to an infection of global distribution causing a range of hepatic and extra-hepatic illness. However, the key proteins playing important parts in the virus infection were still unknown. Understanding the changes of cellular proteins in these cells exposed to HEV is helpful for elucidating molecular mechanisms associated with function alterations of HEV-infected susceptible cells. In the present study, a comparative gel-based proteomic analysis was employed to study the changes in cellular proteins of A549 exposed to HEV in vitro to provide novel information for understanding the functional alterations of A549 induced by HEV infection.
RESULT:
Of 2 585-3 152 protein spots visualized on each gel using silver staining, a total of 31 protein spots were found to be differentially expressed in HEV-infected A549 cells compared with mock-infected A549, including 10 significantly up-regulated protein spots and 21 significantly down-regulated protein spots.
CONCLUSION:
Our work is the first time regarding the proteomic analysis on the cellular responses to HEV infection. This work is helpful for investigating the molecular basis associated with the interaction between HEV and the host cells although more efforts should be required to discover the mechanisms.

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